The New Food Safety Testing Gold Standard: Whole Genome Sequencing

Promoting and Advancing Petroleum Marketing, Retail Grocery, and Convenience Stores in West Virginia

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OMEGA is a West Virginia-based organization dedicated to supporting and advancing the business interests of the state’s petroleum marketing, retail grocery, and convenience store industries.

As a network of over 225 locally owned businesses, OMEGA actively promotes cooperation, advocates for favorable legislation, and provides education and resources to its members. With over 50,000 employees, OMEGA members significantly contribute to the state’s economy, generating more than 10% of all state taxes and supporting local communities through scholarships, charitable donations, and civic involvement.

OMEGA is committed to fostering industry growth while serving West Virginians with essential services like fuel, food, and convenience.

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OMEGA offers three types of membership: Convenience Store Members, Petroleum Marketer Members, and Grocer Members, all of which play a vital role in providing fuel, food, and essentials across West Virginia. As a liaison with state and national agencies, OMEGA works closely with organizations like NACS, PMAA, FMI, and the N.G.A. to advocate on behalf of its members.

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While the Food Safety Modernization Act of 2011 (FSMA) has updated U.S. food safety regulations, an emerging technology, known as whole genome sequencing (WGS), is revolutionizing outbreak investigations to more rapidly identify and prevent pathogenic bacteria from entering our food supply.

By: Adam Friedlander, Specialist, Food Safety and Technical Services, Food Marketing Institute <img src="http://www.fmi.org/images/default-source/blog-images/dna-163466_1280.tmb-large-350-.jpg?sfvrsn=155b726e_1" data-displaymode="Thumbnail" alt="DNA" title="DNA" style="float: right; margin-bottom: 10px; margin-left: 10px;" />While the Food Safety Modernization Act of 2011 (<a href="https://www.fda.gov/Food/GuidanceRegulation/FSMA/default.htm">FSMA</a>) has updated U.S. food safety regulations, an emerging technology, known as <a href="https://www.fmi.org/blog/view/fmi-blog/2017/04/26/whole-genome-sequencing-big-data-generating-a-safer-food-supply">whole genome sequencing</a> (WGS), is revolutionizing outbreak investigations to more rapidly identify and prevent pathogenic bacteria from entering our food supply. Whole genome sequencing is a microbiological testing procedure which determines the order of bases in the genome of an organism – think DNA fingerprint analysis for bacteria. The sample is plated, purified, sheared, copied and sequenced into a computer analyzer to map out the genome. The organism’s identity is discovered through a computer program, which assembles millions of DNA reads into the correct order, similar to a jigsaw puzzle. Once complete, the collected data are entered into private and public databases, known as <a href="https://www.cdc.gov/pulsenet/index.html">PulseNet</a> and <a href="https://www.fda.gov/food/foodscienceresearch/wholegenomesequencingprogramwgs/ucm363134.htm">GenomeTrakr</a>, respectively, where further analysis is required to identify specific pathogenic strains and genetic traits.  In 1996, the Centers for Disease Control and Prevention (<a href="https://www.cdc.gov/">CDC</a>) began using Pulsed Field Gel Electrophoresis (<a href="https://www.cdc.gov/pulsenet/pathogens/pfge.html">PFGE</a>) to investigate outbreaks through PulseNet. Although this technology helped public health officials solve some of America’s most deadly outbreaks, whole genome sequencing produces more accurate, rapid and cost-effective results. For this reason, the CDC has stated that WGS will replace PFGE as “the new PulseNet gold standard for subtyping pathogens that cause foodborne illness” in 2018. According to a <a href="https://wwwnc.cdc.gov/eid/article/17/1/pdfs/p1-1101.pdf">2011 CDC publication</a>, more than 9.4 million incidences of foodborne illness were reported from the 31 major pathogens. Of these cases, 56,000 were hospitalized and 1,350 died from illnesses directly linked to foodborne pathogens. Symptoms of infection from foodborne pathogens range from relatively mild (such as nausea, diarrhea and fever) to life-threatening. While infants, the elderly, pregnant women and persons with weakened immune systems are most susceptible, pathogenic organisms pose tremendous risk to the entire population.   On May 22-23, 2017, the Institute for Food Safety and Health (<a href="https://www.ifsh.iit.edu/sites/ifsh/files/departments/FINAL_WGS_Symposium_050117.pdf">IFSH</a>) hosted a symposium, Whole Genome Sequencing for the Food Industry: Current Procedures, Advances, Obstacles and Solutions, in Burr Ridge, IL. Speakers from government, academia and industry shared cutting-edge research, best practices for environmental sampling, and hurdles associated with analyzing large data sets. This conference demonstrated how government agencies are committed to developing practical policies alongside industry leaders to improve WGS surveillance. It is important to note that WGS will be used in conjunction with other tools by regulatory agencies for outbreak investigations. Focusing resources on proper cleaning and sanitation protocols, good manufacturing practices (GMPs) or good retail practices, and comprehensive food safety training are the most effective strategies to combat foodborne illness. As WGS technology continues to advance, further research is critical to identify bacterial strains and genetic traits, while reducing foodborne illness and outbreaks across the supply chain. For more food safety information, visit <a href="http://www.fmi.org/FoodSafety">www.FMI.org/FoodSafety</a>.

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Supporting West Virginia Communities

OMEGA members play a vital role in West Virginia, supporting communities through substantial tax contributions and a wide-reaching presence across the state. With over 55% of the population living in border counties, our members are dedicated to serving both local and regional needs. Beyond business, we’re proud to give back, having contributed over $3.1 million to children’s charities since 2003, reflecting our commitment to making a positive difference.